| ORIGINAL ARTICLE |
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| Year : 2018 | Volume
: 13
| Issue : 1 | Page : 38-43 |
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Human leukocyte Antigen-B*27 allele subtype prevalence and disease association of ankylosing spondylitis among south indian population
Vikram Haridas1, Praveenkumar Shetty2, M Nirmal Kumar3, KC Vasanthakumar4, Kiran Haridas4, Vitthal Khode5, Anil Bargale2
1 Arthritis Superspeciality Center; Departments of Medicine, SDM College of Medical Sciences and Hospital, Hubli-Dharwad, Karnataka, India
2 Department of Biochemistry, SDM College of Medical Sciences and Hospital, Hubli-Dharwad, Karnataka, India
3 Jeevan Stem Cell Foundation, Chennai, Tamil Nadu, India
4 Arthritis Superspeciality Center, Hubli-Dharwad, Karnataka, India
5 Department of Physiology, SDM College of Medical Sciences and Hospital, Hubli-Dharwad, Karnataka, India
Correspondence Address:
Dr. Praveenkumar Shetty
Department of Biochemistry, SDM College of Medical Sciences and Hospital, Hubali-Dharwad, Karnataka
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/injr.injr_95_17
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Aim: Ankylosing spondylitis (AS) is a chronic inflammatory arthritis mainly affecting articular and extraarticular structures. AS clinical manifestations also involve sacroiliac joints and spine. Genetic factors play a key role in AS susceptibility. AS-associated subtypes of human leukocyte antigen (HLA)-B27 and other HLA-B alleles vary in different ethnic populations. There are no reports of HLA B genotype association to South Indian AS patients. In the current study, we have analyzed the HLA-B genotype association with 105 AS patients and 100 respective controls, we have also verified whether any specific clinical manifestation of AS has any pattern of HLA-B subtype association.
Methods: The patients with AS were diagnosed fulfilling ASAS criteria. Before enrolling the patients, the written informed consent was obtained. The peripheral blood DNA genotyping of HLA-B27 was performed in Applied Biotechnologies 3130/3500 sequencer using SeCore HLA B Class I typing high-resolution kit from Invitrogen.
Results: HLA B27 allele frequency in AS patients (74%) is significantly higher than healthy controls (3%). Most of the earlier studies associated AS with HLA B27 antigen. The current data illustrate that only 21% of AS patients presented HLA B27 antigen. HLA B27:05 and HLA B27:04 are the predominant subtypes. Early-onset of AS manifestations is seen in HLA B27 phenotypes than non-HLA B27 phenotypes. HLA B27 associated AS patients presented more severe axial manifestations such as bilateral sacroiliitis, erosions, and extra-articular features such as uveitis than non-HLA types. Positivity for HLA B27 allele predicts more severe disease course in South Indian patients with AS, similar to that in other populations.
Conclusion: The current study indicates that a majority of South Indian AS patients are associated with HLA-B*27 alleles. In addtion we found that HLA-B*27 associated AS patients presented with more severe axial manifestations.
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