Indian Journal of Rheumatology

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 9  |  Issue : 4  |  Page : 167--177

Deflazacort alleviate pro-inflammatory cytokines expression, oxidative stress and histopathological alterations in collagen induced arthritis in Wistar rats


Neha, MdMeraj Ansari, Haider A Khan 
 Heavy Metal and Clinical Toxicology Laboratory, Department of Medical Elementology and Toxicology, Jamia, Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India

Correspondence Address:
Haider A Khan
Heavy Metal and Clinical Toxicology Laboratory, Department of Medical Elementology and Toxicology, Jamia, Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062
India

Objectives: Glucocorticoids are important and frequently used class of anti-inflammatory drugs. Deflazacort (DFZ) is a glucocorticoid and an oxazolone derivative of prednisolone. As it has high anti-inflammatory and immunosuppressive potency, we studied inhibitory effect of DFZ on mediators regulating the pro-inflammatory response and oxidative stress induced in arthritis. Methods: Female Wistar rats were immunized intradermally by collagen type II to induce collagen induced arthritis. Arthritic rats were treated with DFZ orally (6 mg kg-1 body weight) until 28 days after the onset of clinical symptoms of disease. The effect of DFZ treatment in the rats was monitored by clinical scoring, biochemical parameters, immu- nohistochemistry and histopathological evaluation. Results: Deflazacort significantly decreased the level of articular elastase, nitric oxide and lipid peroxidation whereas significantly increased the activity of catalase, superoxide dismutase and glutathione. Deflazacort down-regulated expression of pro-inflammatory molecules like TNF-a and COX-2. Histopathological evaluation revealed significant reduc- tion of synovial hyperplasia and cellular infiltration in synovial membrane in DFZ treated group as compared to the diseased group. Conclusions: This suggests that DFZ significantly down-regulates the expression of pro-in- flammatory molecules such as TNF-a and COX-2 and alleviates the oxidative stress which make it a viable therapeutic option for treatment of arthritis.


How to cite this article:
Neha, Ansari M, Khan HA. Deflazacort alleviate pro-inflammatory cytokines expression, oxidative stress and histopathological alterations in collagen induced arthritis in Wistar rats.Indian J Rheumatol 2014;9:167-177


How to cite this URL:
Neha, Ansari M, Khan HA. Deflazacort alleviate pro-inflammatory cytokines expression, oxidative stress and histopathological alterations in collagen induced arthritis in Wistar rats. Indian J Rheumatol [serial online] 2014 [cited 2020 Oct 23 ];9:167-177
Available from: https://www.indianjrheumatol.com/article.asp?issn=0973-3698;year=2014;volume=9;issue=4;spage=167;epage=177;aulast=Neha,;type=0