Indian Journal of Rheumatology

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 12  |  Issue : 2  |  Page : 86--93

Immune modulation effects of curcumin in pristane-induced lupus mice


Handono Kalim1, Kusworini Handono2, Takhta Khalasha3, Mirza Zaka Pratama3, Tri Wahyudi Iman Dantara3, Ayu Pramitha Wulandari3, Fahimma Albinsaid3, Sofi Nur Fitria3, Muhammad Vardian Mahardika3 
1 Department of Internal Medicine, Rheumatology and Immunology Division, Faculty of Medicine Brawijaya University, Saiful Anwar General Hospital, Malang, East Java 65111, Indonesia
2 Department of Clinical Pathology, Faculty of Medicine Brawijaya University, Saiful Anwar General Hospital, Malang, East Java 65111, Indonesia
3 Department of Biomedical Sciences, Faculty of Medicine Brawijaya University, Saiful Anwar General Hospital, Malang, East Java 65111, Indonesia

Correspondence Address:
Handono Kalim
Department of Internal Medicine, Rheumatology and Immunology Division, Faculty of Medicine Brawijaya University, Saiful Anwar General Hospital, Jalan JA Suprapto No. 2, Malang, East Java 65111
Indonesia

Background: Curcumin, a polyphenolic compound derived from food spice turmeric has been widely used in Asian traditional medicine for its medicinal properties as antitumor, antioxidant, and anti-inflammatory properties. Meanwhile, intraperitoneal (i.p.) injection of the hydrocarbon oil pristane into normal mice leads to a lupus-like autoimmune syndrome. We aimed to investigate the effects of curcumin on systemic lupus erythematosus (SLE) clinical manifestation, adaptive immune system components, proinflammatory cytokines, and autoantibody production in pristane-induced lupus mice. Methods: Fifty female BALB/c mice, 6–8 weeks old were divided into 2 groups: Forty mice received a single i.p. injection of 0.5 cc pristane for lupus induction and ten mice as healthy controls. Starting at 16 weeks after injection, forty pristane-induced lupus mice were divided into four groups based on doses of curcumin received intragastrically: 0, 12.5, 50, and 200 mg/kg bw/day daily for 16 weeks. At 32 weeks after injection, all of mice were assessed for arthritis score, proteinuria level, body weights, adaptive immune system components (Th1, Th2, Th17, and Treg percentages) from spleen using flow cytometry; proinflammatory cytokines and autoantibody production, including interleukin-6 (IL-6), interferon-alpha (IFN-α), and antinuclear antibody (ANA) from serum using enzyme-linked immunosorbent assay. Results: Arthritis score and proteinuria level were decreased in curcumin-treated mice. However, body weights were not significantly different between the groups. The decreased of Th1, Th2, and Th17 percentages were seen after treatment with 200 mg/kg bw/day of curcumin (P = 0.031,P = 0.017, andP = 0.005, respectively). However, only slight increase of Treg percentages was seen after curcumin treatment. Treatment with 200 mg/kg bw/day of curcumin decreased serum IL-6 and IFN-α levels (P = 0.007 andP = 0.003). Furthermore, ANA levels were also decreased significantly after treatment with 200 mg/kg bw/day of curcumin (P = 0.013). Conclusion: Our findings suggested that curcumin could prove useful as a therapeutic intervention in SLE.


How to cite this article:
Kalim H, Handono K, Khalasha T, Pratama MZ, Dantara TW, Wulandari AP, Albinsaid F, Fitria SN, Mahardika MV. Immune modulation effects of curcumin in pristane-induced lupus mice.Indian J Rheumatol 2017;12:86-93


How to cite this URL:
Kalim H, Handono K, Khalasha T, Pratama MZ, Dantara TW, Wulandari AP, Albinsaid F, Fitria SN, Mahardika MV. Immune modulation effects of curcumin in pristane-induced lupus mice. Indian J Rheumatol [serial online] 2017 [cited 2022 Oct 4 ];12:86-93
Available from: https://www.indianjrheumatol.com/article.asp?issn=0973-3698;year=2017;volume=12;issue=2;spage=86;epage=93;aulast=Kalim;type=0